empty vector Search Results


cre lentiviral plasmids  (Addgene inc)
91
Addgene inc cre lentiviral plasmids
Cre Lentiviral Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmscv pig vector  (Addgene inc)
93
Addgene inc pmscv pig vector
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Pmscv Pig Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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starr seq luciferase validation vector scp1 empty  (Addgene inc)
91
Addgene inc starr seq luciferase validation vector scp1 empty
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Starr Seq Luciferase Validation Vector Scp1 Empty, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/STARR-seq+luciferase+validation+vector_SCP1_empty+(Plasmid+%2399299)/pmc07044160-279-3-7
Average 91 stars, based on 1 article reviews
starr seq luciferase validation vector scp1 empty - by Bioz Stars, 2026-09
91/100 stars
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empty vector  (Addgene inc)
90
Addgene inc empty vector
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/CMV500+empty+vector+(Plasmid+%2333348)/pm36026443-242-25-30
Average 90 stars, based on 1 article reviews
empty vector - by Bioz Stars, 2026-09
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cre recombination enzyme  (Addgene inc)
93
Addgene inc cre recombination enzyme
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Cre Recombination Enzyme, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/Puro%2ECre+empty+vector+(Plasmid+%2317408)/pm40585984-261-3-14
Average 93 stars, based on 1 article reviews
cre recombination enzyme - by Bioz Stars, 2026-09
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luciferase validation vector ori empty plasmid  (Addgene inc)
93
Addgene inc luciferase validation vector ori empty plasmid
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Luciferase Validation Vector Ori Empty Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/STARR-seq+luciferase+validation+vector_mP_empty+(Plasmid+%2399298)/pmc09712620-220-10-16
Average 93 stars, based on 1 article reviews
luciferase validation vector ori empty plasmid - by Bioz Stars, 2026-09
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pfugw h1 empty vector  (Addgene inc)
93
Addgene inc pfugw h1 empty vector
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Pfugw H1 Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/pFUGW-H1+empty+vector+(Plasmid+%2325870)/pmc06444434-36-11-14
Average 93 stars, based on 1 article reviews
pfugw h1 empty vector - by Bioz Stars, 2026-09
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alexander stark  (Addgene inc)
93
Addgene inc alexander stark
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Alexander Stark, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/STARR-seq+luciferase+validation+vector_ORI_empty+(Plasmid+%2399297)/pmc12780183-476-10-12
Average 93 stars, based on 1 article reviews
alexander stark - by Bioz Stars, 2026-09
93/100 stars
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luc cre lentiviral plasmid  (Addgene inc)
93
Addgene inc luc cre lentiviral plasmid
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Luc Cre Lentiviral Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/Luc%2ECre+empty+vector+(Plasmid+%2320905)/10__1158_slash_2159___8290__cd___13___0346-189-2-7
Average 93 stars, based on 1 article reviews
luc cre lentiviral plasmid - by Bioz Stars, 2026-09
93/100 stars
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lentivirus plasmids  (Addgene inc)
92
Addgene inc lentivirus plasmids
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Lentivirus Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/pLOVE+empty+vector+(Plasmid+%2315948)/pmc03642324-325-2-7
Average 92 stars, based on 1 article reviews
lentivirus plasmids - by Bioz Stars, 2026-09
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cmv566 empty vector  (Addgene inc)
90
Addgene inc cmv566 empty vector
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Cmv566 Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/CMV566+empty+vector+(Plasmid+%2333349)/pm25263564-224-6-12
Average 90 stars, based on 1 article reviews
cmv566 empty vector - by Bioz Stars, 2026-09
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pet empty polycistronic destination vector 2e  (Addgene inc)
86
Addgene inc pet empty polycistronic destination vector 2e
The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the <t>pMSCV</t> <t>PIG</t> vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.
Pet Empty Polycistronic Destination Vector 2e, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet empty polycistronic destination vector 2e - by Bioz Stars, 2026-09
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Image Search Results


The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the pMSCV PIG vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.

Journal: International Journal of Molecular Sciences

Article Title: Ocular Albinism Type 1 Regulates Melanogenesis in Mouse Melanocytes

doi: 10.3390/ijms17101596

Figure Lengend Snippet: The overexpression of OA1 affects the melanin content in an MITF-dependent fashion. Mice OA1 was PCR amplified and then subcloned into the pMSCV PIG vector with Xho I and EcoR I restriction sites. The pMSCV-OA1-GFP plasmid or empty vector was transfected into the mouse melanocytes. The cells were harvested to extract total RNA and total protein. The expression levels of OA1 were qualitatively and quantitatively analyzed by qRT-PCR and Western blot. ( A ) Melanin contents in melanocytes transfected with OA1 , as well as the control; ( B – D ) transfection efficiency of OA1 in mouse melanocytes; ( E – G ) OA1 overexpression influences microphthalmia-associated transcription factor ( MITF ) mRNA and protein levels in mouse melanocytes. Data are shown as the mean ± standard errors ( n = 3 each), * p < 0.05, ** p < 0.01.

Article Snippet: The mice OA1 gene was PCR amplified and then was cloned into the pMSCV PIG vector (Addgene, Cambridge, MA, USA) with Xho I and EcoR I restriction sites.

Techniques: Over Expression, Amplification, Plasmid Preparation, Transfection, Expressing, Quantitative RT-PCR, Western Blot